The Lunch Talks are back! After 2 years of absence, the DKFZ Postdoc Network and BioMed X are proud to announce the relaunch of the Lunch Talks, a space to discuss science and data in a relaxed environment and with tasty snacks.
We look forward to seeing you there on November 17th. Please note that spaces are limited and registration is mandatory. If you register and cannot make it, please cancel your registration at least 48 hours in advance.
Lunch Talk by Claudia Kutter - presented by BioMed X and the DKFZ PostDoc Network.
"Yin Yang of coding and noncoding transcriptomes in healthy and diseased liver cells"
by Claudia Kutter, SciLifeLab, Karolinska Institute.
Host: Stefania Del Prete
In the “Lunch Talk Series", group leaders from BioMed X Institute and the PostDoc Network of the German Cancer Research Center invite renowned life scientists to share their research and foster scientific exchanges.
When: Thursday, 17th November 2022, 11:00 am
Where: DKFZ Communication Center K1-K2 Rooms, Im Neuenheimer Feld 280, Heidelberg
RNA-binding proteins (RBPs) play crucial roles in cancer and accumulating evidence suggests functionality through long non-coding RNAs (lncRNAs). We curated a list of ~2.300 RNA binding proteins in human cells, which are highly expressed and negatively affect patient survival in liver cancer (HCC). Upon knockdown of the top ten upregulated RBPs and subsequent transcriptome analysis, we identified differentially expressed lncRNAs. CRISPRa-mediated overexpression of four lncRNAs had major effects on the liver cancer cell phenotype and transcriptome. Further investigation of four RBP-lncRNA pairs revealed involvement in distinct regulatory processes. I will present more details of the most noticeable RBP-lncRNA connection affecting lipid metabolism, whereby the non-canonical RBP CCT3 regulated LINC00326 in a chaperonin-independent manner. Remarkably, perturbation of the CCT3-LINC00326 regulatory network led to decreased lipid accumulation and increased lipid degradation in cellulo as well as diminished tumor growth in vivo. In sum, our work revealed that RBP gene expression is perturbed in HCC and identified additional RBP functions exerted beyond their tasks under normal physiological conditions, which can be stimulated or intensified via lncRNAs and are relevant for tumor growth.
If time permits, I will elaborate on methodological advances to study RBP-RNA interactions as well as detection of unexpected CRISPR/Cas9 on- and off-targeting events.
Dr. Claudia Kutter, SciLifeLab, Karolinska Institute
Dr. Claudia Kutter received her PhD from the University of Basel, Switzerland. During her PhD, she worked on small RNA-mediated regulation of stem cell differentiation at the Friedrich Miescher Institute (Basel, Switzerland). As a postdoctoral researcher, Claudia joint Cancer Research UK and the University of Cambridge (Cambridge, UK) to study the evolution of noncoding RNAs using next-generation sequencing methods. Claudia became a SciLifeLab and Wallenberg fellow at the Karolinska Institute (Department of Microbiology, Tumor and Cell Biology) in 2016. She is leading her independent research group at the Science for Life Laboratory (SciLifeLab) in Stockholm.
In her talk, Claudia will present her recent work in deciphering genome functionality in healthy and diseased cells, in particular how coding and noncoding transcriptomes are entwined.
Please note that spaces are extremely limited so registration is mandatory. If you register and cannot make it, please cancel your registration at least 48 hours in advance so someone from the waiting list will be invited.
Communication Center, K1+K2
Im Neuenheimer Feld 280, Heidelberg
The Postdoc Network Committee and the BioMed X Institute