Speakers
Description
Targeted metabolomics workflows are often constrained by limited analyte coverage and high per-sample costs of commercial kit-based assays, leading to incomplete metabolic insights. Here, we present a comprehensive and cost-efficient targeted metabolomics platform that combines broad analyte coverage with high throughput across diverse biological matrices. Utilizing the MetaSci Complete Library - encompassing >1,200 metabolites spanning central carbon, lipid, amino acid, and nucleotide pathways - our method can be tailored from focused analyses of specific compound classes to broad screening of several hundred metabolites per sample. Analytical measurements employ a QTRAP 6500+ mass spectrometer with dual HILIC and reversed-phase chromatography to accommodate polar and nonpolar analytes. We demonstrate the workflow’s versatility and robustness through metabolic profiling of mammalian tissues (e.g., mouse liver), plant tissues (e.g., Arabidopsis thaliana leaves), biofluids (e.g., human plasma) and cultured cells (e.g., cancer cell lines). Each matrix yields detection and quantification of hundreds of metabolites within a single analytical experiment. Compared to commercial kit-based assays or untargeted approaches, our platform reduces per-sample costs significantly while maintaining high sensitivity and reproducibility. This user-friendly workflow offers a scalable and modular solution for targeted metabolomics in academic and clinical research settings.
| Research type | Other |
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